Porcellio scaber BK24

Sample information

Picture
Photos by: Benoit K.
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda Porcellionidae Porcellio Porcellio scaber

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Line 24: no CO1-Band, no W-Band

in the same series – Drosophila positive control Vanderbilt worked well, see line 26

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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