Wolbachia Project- SB

September 7, 2023

Sample information
Picture
Photos by: Sephora B.
Location
Collection date
Captive / Cultivated? Wild-caught
Group South Plainfield High School
Observations

Male/Female specify
Collected by the
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be postive for Wolbachia.
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