Mosquito – RudraP

September 7, 2023

Sample information
Picture
Photos by: Rudra Patel
Location
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

This mosquito is a female because it has similar features like other female mosquitoes.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Qiagen blood+tissue kit – with modification P2 for ATL + N3 for AL

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The Wolbachia positive is no because not all mosquitoes had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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