Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | South Plainfield High School |
| Observations | This mosquito is a female because it has similar features like other female mosquitoes. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | Qiagen blood+tissue kit – with modification P2 for ATL + N3 for AL |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The Wolbachia positive is no because not all mosquitoes had Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culex was found to be negative for Wolbachia. |
Water Boatman