Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/18/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Large, yellow, 6 long skinny yellow legs, 2 wings. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Tipulidae Nephrotoma |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | Other |
| DNA stain | Other |
| Gel images |
|
| Protocol notes |
|
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The first time we completed the gel electrophoresis lab, I accidentally didn’t add some of the primers, so nothing was showing up. However, we later redid it correctly, and our results displayed no Wolbachia, and the controls worked. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Nephrotoma was found to be negative for Wolbachia. |
Thrip – Phlaeothripidae Leptothrips sp. 21
MACALF24-SB Dermaptera
Thrip – Phlaeothripidae Leptothrips sp. 19