Crane Fly

September 7, 2023

Sample information
Picture
Photos by: Kayden V.
Location
Collection date 09/18/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Large, yellow, 6 long skinny yellow legs, 2 wings.
Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae Nephrotoma

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer Other
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The first time we completed the gel electrophoresis lab, I accidentally didn’t add some of the primers, so nothing was showing up. However, we later redid it correctly, and our results displayed no Wolbachia, and the controls worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Nephrotoma was found to be negative for Wolbachia.
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