Papilio polyxenes caterpillar

November 2, 2022

Sample information
Picture
Photos by: Shreyan M.
Location
Collection date 09/09/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Moves slowly, eats once a day
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Papilionidae Papilio Papilio polyxenes

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Other
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TTGAGCAAGTATATTAGGAACTTCACTTAGCTTATTAATTCGTACTGAATTAGGAACACCAGGTTCTTTAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATGCCTATTATAATTGGAGGATTTGGAAATTGATTAATTCCTTTAATATTAGGAGCCCCTGATATAGCTTTCCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCTTCATTAACTCTTTTAATTTCAAGAATAATCGTAGAAAATGGAGCTGGAACCGGTTGAACCGTTTACCCCCCTCTCTCTTCTAATATTGCCCATGGAAGAAGATCAGTAGATTTAGTTATTTTTTCTTTACATTTAGCAGGAATTTCCTCAATTTTAGGAGCAATTAATTTTATTACAACAATTATTAATATACGTATTAATAATATATCATTTGATCAAATACCTTTATTTGTTTGAGCCGTTGGAATTACAGCTTTATTATTACTTCTTTCTCTACCAGTTTTAGCTGGAGCTATTACTATATTACTAACAGATCGAAATCTAAATACTTCTTTTTTTGATCCTGCAGGAGGAGGAGATCCTATTTTATATCAACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Papilio polyxenes was found to be negative for Wolbachia.
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