Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/10/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Captured underneath a moist leaf pile. No other ants were spotted nearby. Caught around 5pm in the afternoon and immediately put under refrigeration after photography. 6 legs, head/abdomen/thorax insect. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | A strong positive band was revealed for the ant, but none of the other 3 arthropods. No noticeable errors were made during electrophoresis. We used a 10kb DNA ladder |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | No errors were made noticeable during or after the procedure. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
>Mod B1-WSPECF.scf (352 bp) GGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGT AATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATG GAGTGGGCTACACACGTGCTACAATGGTGGCTATAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCAT CTCANTTCGGATTGTACTCTGCAACTCNAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAA TACGTTCTCGGGTCTTGTACACACTGCCCGTC
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
>Mod B1-CO1F.scf (592 bp) AATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCA ATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTT TTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATC GATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAA ATACCTTCCATAGNGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCA ATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTANTATGATCTAT TCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATC TTAATACTTCATTTTTCGATCCCTCGGGAGGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Water Boatman