Spider

November 23, 2022

Sample information
Picture
Photos by: Finley T., Sophia C., Krishna L
Location
Collection date 06/18/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Spider was caught on my bathroom wall. it was caught mid-June; the outside temperatures were in the 80’s, the inside temperature was mid-60’s.
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The only thing I would redo for our lab would be the injection of the DNA/Controls into the gel. During this process we would occasionally go to deep or puncture the gel.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneae was found to be negative for Wolbachia.
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