Spider

Sample information
Picture	Photos by: Finley T., Sophia C., Krishna L
Location
Collection date	06/18/2022
Captive / Cultivated?	Wild-caught
Group	Edmund Burke School
Observations	Spider was caught on my bathroom wall. it was caught mid-June; the outside temperatures were in the 80’s, the inside temperature was mid-60’s.
Putative identification	Arthropoda Chelicerata Arachnida Araneae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	The only thing I would redo for our lab would be the injection of the DNA/Controls into the gel. During this process we would occasionally go to deep or puncture the gel.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Araneae was found to be negative for Wolbachia.

The Wolbachia Project Database