Common Oak Moth

Sample information

Photos by: Finley T., Sophia C., Krishna L
Collection date 07/09/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Erebidae Phoberia Phoberia atomaris


Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phoberia atomaris was found to be negative for Wolbachia.
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