Rhino Beetle

Sample information
Picture	Photos by: Finley T., Sophia C., Krishna L
Location
Collection date	08/30/2022
Captive / Cultivated?	Wild-caught
Group	Edmund Burke School
Observations	I collected this beetle in a corn field in Maryland in late august, it was about 70 degrees Fahrenheit. It was caught in early afternoon. It was the only beetle in the immediate area that I could see.
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Xyloryctes Xyloryctes jamaicensis
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Gonads dissected
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	10kb DNA ladder used. The only thing I would redo for our lab would be the injection of the DNA/Controls into the gel. During this process we would occasionally go to deep or puncture the gel. 10kb DNA ladder used.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Xyloryctes jamaicensis was found to be negative for Wolbachia.

The Wolbachia Project Database