Rhino Beetle

November 23, 2022

Sample information
Picture
Photos by: Finley T., Sophia C., Krishna L
Location
Collection date 08/30/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

I collected this beetle in a corn field in Maryland in late august, it was about 70 degrees Fahrenheit.  It was caught in early afternoon. It was the only beetle in the immediate area that I could see.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Xyloryctes Xyloryctes jamaicensis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

10kb DNA ladder used.

The only thing I would redo for our lab would be the injection of the DNA/Controls into the gel. During this process we would occasionally go to deep or puncture the gel. 10kb DNA ladder used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Xyloryctes jamaicensis was found to be negative for Wolbachia.
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