Spider

Sample information

Picture
Photos by: Grace W.
Location
Collection date 09/15/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

I found this Spider in my house near Washington, D.C. (which is kept at a temperature of approximately 72 degrees F). I collected it in the morning, around 7:30 AM, and the outdoor temperature that day was in the 70s (F). The spider is small/medium sized with red/brown coloring, long legs, and bulging circular abdomen. I later found it to be a pregnant female. It had low energy and did not move a lot. It also had a tendency to hide in dark areas and play dead when frightened.

Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: I made sure to crush the arthropod really well. I dissected the abdomen out, and did not include the exoskeleton.

Gel electrophoresis lanes for CO1 arthropod PCR:

1- 10kb DNA ladder.

2-The second well is my earwig (E-1)

3-The third well is my bee (E-2)

4- The fourth well is my spider (E-3)

5- The fifth well is my cricket (E-4)

6- The sixth well is my (+) arthropod control

7- The seventh well is my (-) arthropod control

8- The eight well is my DNA control

9- The ninth well is my H20 control

Analysis: My controls worked, but sample E-1 did not have a band. There could have been a problem with the primer sticking.

Gel electrophoresis lanes for Wolbachia PCR:

1- 10kb DNA ladder.

2-The second well is my earwig (E-1)

3-The third well is my bee (E-2)

4- The fourth well is my spider (E-3)

5- The fifth well is my cricket (E-4)

6- The sixth well is my (+) arthropod control

7- The seventh well is my (-) arthropod control

8- The eight well is my DNA control

9- The ninth well is my H20 control

Analysis: My controls worked, so I am confident that there was no contamination.

 

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected. I did not have any problems with the protocol, and I was not surprised by any bands on the gel. Therefore, I am confident that my arthropod is not infected with Wolbachia.

Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Araneae was found to be negative for Wolbachia.
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