Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | |
| Putative identification | Arthropoda Myriapoda Diplopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diplopoda was found to be negative for Wolbachia. |
Water Boatman