Millipede

November 23, 2022

Sample information
Picture
Photos by: Finley T., Sophia C., Krishna L
Location
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations
Putative identification Arthropoda Myriapoda Diplopoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

When we completed the PCR test, there weren’t any lines in an unexpected value, therefore there was no detected contamination in our tests. Our positive controls worked, and our negative controls showed no signs of contamination. The DNA ladder is a little weak, so we think we punctured the gel when prepping for Gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diplopoda was found to be negative for Wolbachia.
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