|Captive / Cultivated?||Wild-caught|
|Group||Edmund Burke School|
We weren’t the ones who collected the arthropod, so we aren’t sure the circumstances under which it was caught.
|Putative identification||Hexapoda Insecta Lepidoptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
DNA extraction: The whole moth was used.
Gel electrophoresis lanes:
Lane 1: 10kb DNA Ladder
We know the results for the arthropod and Wolbachia tests for the A-4 sample are reliable because the sample tested positive for arthropod DNA and the results have no contamination because our controls tested correctly.
|Explanation of confidence level||
The sample tested negative for Wolbachia. Our confidence level is medium. There is a small chance that our results aren’t all completely accurate, because there was an error with our A-2 sample during one of our procedures that resulted in it testing negative for the CO1 gene. Even though the A-2 sample was cut from our results, it still goes to show that human error is always possible.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
ATATTAGGAACTTCTTTGAGATTATTAATTCGAGCTGAATTAGGAAACCCAGGATCTTTAATTGGAGATGATCAAATTTA TAATACAATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATT GATTAGTTCCTTTAATACTAGGAGCCCCAGATATAGCATTCCCACGAATAAATAATATAAGTTTTTGACTATTACCCCCA TCATTAACTTTATTAATTTCAAGAAGAATTGTCGAAAATGGAGCTGGAACAGGATGAACTGTTTACCCTCCCCTATCTTC TAACATTGCTCACGGAGGTGGATCAGTAGATTTAGCAATTTTTTCTTTACATTTAGCTGGAATTTCCTCAATTCTAGGAG CTGTAAATTTTATTACAACAGTAATTAATATACGGACCATTGGAATATCCTTTGATCGTATACCTTTATTTGTTTGATCT GTAGCAATTACBLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Lepidoptera was found to be negative for Wolbachia.|