|Captive / Cultivated?||Wild-caught|
|Group||Edmund Burke School|
It was a butterfly with bands of red and white on the edges of its wings. Its base color is black and had two antennas. Was caught in summer and is was very warm. Was caught in the middle of the day.
|Putative identification||Hexapoda Insecta Lepidoptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
DNA Extraction: we crushed the abdomen of the bugs well.
Gel electrophoresis lanes for arthropod
|Explanation of confidence level||
All of our controls in both the Arthropod and Wolbachia test worked perfectly. Therefore we can assume from the results of the controls that there was no contamination or mistakes.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
GAATAGTAGGTACTTCTTTAAGNNTNNTNATTCGAACTGAATTAGGNNATCCTGGATCATTAATTGGAGATGANCAAATT TATAATNCTATNGTNACTGCTCATGCTTTTATTATAATTTTTTTTATNGTAATNCCAATTATAATTGGAGGNTTTGGAAA NTGANTAGTNCCTTTNATANTAGGNGCTCCTGATATAGCTTTCCCCCGAATAAATAANATAAGATTTTGACTNNTNCCCC CCTCTTTAACTTTACTNATNGCAAGNAGAATTGTNGAAAATGGAGCNGGAACAGGATGAACNGTTTANCCCCCNCTNTCN TCNAATATNGCTCATNGAGGATCNTCTGTNGANTTAGCTATNTTTTCTNTNCATTTAGCAGGAATTTCNTCAATTTTAGG AGCAATTAATTTTATTACAACTATNATTAATATNCGANNTNNTGGAATNNCNTTTGANCAAATATCTTTATTTNTTTGAT CTGTNGGNATTACTGCNNTTTTATTNTTATTATCATTACCTGTNNTNGCNGGAGNNATNACTATANTTTTAANNGATCNN AAATNTAAATANTTCNTTNTTTGACCCTGCCBLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Lepidoptera was found to be negative for Wolbachia.|