Maple Zale Moth

November 21, 2022

Sample information
Picture
Photos by: Stella T
Location
Collection date 07/21/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Brown with speckles. Caught in the afternoon and it was warm.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: we crushed the abdomen of the bugs well.

Gel electrophoresis lanes for arthropod
1- 10kb DNA Ladder
2- lane two is sample A-1
3- lane three is sample A-2
4- lane four is sample A-3
5- lane five is sample A-4
6- lane six is (+) arthropod control
7- lane seven is (-) arthropod control
8- lane eight is (+) DNA control
9- lane nine is the water control

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked. as well as the arthropod band showed up which means that our DNA extraction was successful.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AGTTGGAACTTCTTTAAGTTTATTAATTCGAGCTGAATTAGGTAACCCCGGCTCTTTAATTGGAGATGATNAAATTTATA ATACTATTGTTACAGCACATGCTTTCATTATAATTTNTTTTATAGTTATACCTATCATAATTGGAGGATTCGGTAATTGA TTAGTTCCATTAATATTAGGAGCTCCTGATATAGCTTTCCCCCGAATAAATAATATAAGTTTTTGATTATTACCTCCTTC TTTAACTCTTTTAATTTCAAGAANAATTGTAGAAAATGGNGCTGGTACAGGATGAACAGTTTACCCCCCTCTATCATCGA ATATTGCCCATAGAGGTAGTTCAGTTGATTTAGCTATTTTTTCATTGCATTTAGCTGGTATTTCATCAATTTTAGGAGCC ATTAACTTTATTACAACAATTATTAACATACGATTAAATAATTTAATATTTGATNAAATACCTTTATTTGTTTGAGCTGN AGGAATTACCGCTTTTCTTCTTTTATTATCTCTTCCAGTTTTAGCAGGAGCTATTACTATATTATTAACTGATCGTAANT TAAATACTTCTTTTTTTGATCCTGCAGGAGGNGGANATCCTATTTNATACCAACATTTATTTTGATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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