|Captive / Cultivated?||Wild-caught|
|Group||Edmund Burke School|
Primarily black insects with yellow accents on their abdomen. Thorax contained a stinger. Found on flowers. has wings. Caught midday at roughly 70 degrees F.
|Putative identification||Arthropoda Hexapoda Insecta Hymenoptera Apidae Apis Apis mellifera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
Followed the protocol provided by The Wolbachia Project with no notable errors. We used a 10 kb DNA ladder.
|Explanation of confidence level||
DNA appeared correctly in both Gel Electrophoresis labs and all controls behaved as expected.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
|Summary||The Apis mellifera was found to be negative for Wolbachia.|