Apis mellifera ligustica

Sample information

Picture
Location
Collection date 09/17/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Primarily black insects with yellow accents on their abdomen. Thorax contained a stinger. Found on flowers. has wings. Caught midday at roughly 70 degrees F.

Putative identification Hexapoda Insecta Hymenoptera Apidae Apis Apis mellifera

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed the protocol provided by The Wolbachia Project with no notable errors. We used a 10 kb DNA ladder.

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

DNA appeared correctly in both Gel Electrophoresis labs and all controls behaved as expected.

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Apis mellifera was found to be negative for Wolbachia.
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