Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Primarily black insects with yellow accents on their abdomen. Thorax contained a stinger. Found on flowers. has wings. Caught midday at roughly 70 degrees F. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Apidae Apis Apis mellifera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed the protocol provided by The Wolbachia Project with no notable errors. We used a 10 kb DNA ladder. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | DNA appeared correctly in both Gel Electrophoresis labs and all controls behaved as expected. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Apis mellifera was found to be negative for Wolbachia. |
Water Boatman