Peleteria iterans- Horsefly

April 27, 2023

Sample information
Picture
Photos by: Chase H.
Location
Collection date 09/05/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Caught on human leg flying by the dry dirt pathway in the hot sun.

Description: Dark coloring, transparent wings, lighter abdomen than thorax.

Body length: 1.2 cm.

Found by Lola Ahlstrom
Putative identification Arthropoda Hexapoda Insecta Diptera Tachinidae Peleteria Peleteria iterans

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
AGGAACATCTTTAAGAATTTTAGTGCGAGCTGAATTAGGACATCCAGGTGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCATTTGTAATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTAGGAGCCCCTGATATAGCTTTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCACCTGCATTAACTTTATTACTTGTAAGAAGAATAGTGGAAAATGGAGCTGGAACAGGATGAACTGTTTACCCTCCATTATCATCTAATATTGCACATGGAGGAGCTTCTGTAGACTTAGCAATTTTTTCTTTACATTTAGCAGGAATTTCTTCTATTCTAGGAGCTGTAAATTTTATTACAACTGTTATTAATATACGATCTGTAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTAGTTATTACAGCATTATTATTACTTTTATCATTACCAGTATTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGAGGAGATCCTATTTTATATCAACATTTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Peleteria iterans was found to be negative for Wolbachia.
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