Camponotus vicinus- Carpenter Ant

April 27, 2023

Sample information
Picture
Photos by: Chase Hartzell and Heather Ahlstrom
Location
Collection date 09/05/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Carpenter Ant/found under nesting soil. Found under a rock surrounded by dry dirt and leaves. Many ants were found at the time, and we selected one out of the group.

Description: Black head and abdomen with a light-colored yellow orange thorax.

Body length: 1.2 cm. Found by Zander Brooks.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus vicinus

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
TATAAGAATAATTATTCCCTTAGAACTAGGATCCACTAACTCACTAATCATAAATGATCAAACTTTTAATACTATTGTTACAAGACATGCTTTTATCATAATCTTTTTTATAGTTATGCCCTTCATAATTGGGGGATTTGGTAACTTCTTACTCCCCCTAATACTCGGATCTCCTGATATAGCCTATCCCCGTATAAACAACATAAGATTTTGATTACTCCCCCCATCAATCTCCCTCCTAATTCTAAGAAATTTTATTAATGAGGGATCTGGAACAGGTTGAACTATTTATCCCCCCTTAGCGTCTAATACCTTCCATAGGGGTTCATCTATTGATCTAACCATCTTCTCCCTTCNTATTGCTGGTATGTCATCAATTCTTGGAGCANTTAATTTTATTTCAACAATTATAAATATACATAATACTAATATCTCATTAGATAAAATTCCCCTCTTANTTTGATCTATTCTTATCACAGCAATCCTTCTCCTCCTATCCCTCCCCGTCCTTGCCGGANCTATTACNATATTATTAACAGATCGAAATCNTAATACTTCCTTCTTCGATCCATCTGGTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus vicinus was found to be negative for Wolbachia.
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