Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Stink Beetle- underneath large rock in brush moist ground Description: Big shiny black large thorax. Body Length: 2.28 cm. Found by Chase Hartzell |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Carabidae Carabus Carabus taedatus |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TACTTCTCTAAGAATACTAATTCNAGCTGAATTGGGAAATCCCGGATCCTTAATTGGAGATGATCAAATTTATAATGTTATTGTAACAGCCCATGCTTTCGTAATAATTTTTTTCATAGTTATACCTATTATAATTGGGGGATTTGGAAATTGATTAGTGCCACTAATATTAGGAGCACCAGATATAGCCTTCCCTCGAATAAATAACATGAGATTTTGACTTTTACCCCCTTCCTTGACCCTTCTCCTAATAAGTAGAATAGTTGAAAAAGGGGCAGGGACAGGTTGAACAGTTTACCCTCCGCTGTCATCAGGAATCGCTCACAGAGGGGCATCAGTGGACCTAGCTATTTTTAGACTTCATTTAGCAGGAATTTCATCAATTTTAGGAGCAGTAAATTTTATTACAACAATTATTAATATACGATCAGTAGGGATAACATTTGATCGAATACCCTTATTTGTATGATCAGTAGGAATTACAGCATTATTATTATTACTATCTTTACCAGTTTTAGCTGGAGCTATTACTATACTTCTAACAGATCGAAAATTTAAATACTTCTTTTTTTGATCCTGCCGGAGGGGGAGATCCTATTCTTTATCAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Carabus taedatus was found to be negative for Wolbachia. |
Water Boatman