Carabus taedatus- Beetle

April 27, 2023

Sample information
Picture
Photos by: Chase Hartzell and Heather Ahlstrom
Location
Collection date 09/05/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Stink Beetle- underneath large rock in brush moist ground

Description: Big shiny black large thorax.

Body Length: 2.28 cm. Found by Chase Hartzell
Putative identification Arthropoda Hexapoda Insecta Coleoptera Carabidae Carabus Carabus taedatus

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
TACTTCTCTAAGAATACTAATTCNAGCTGAATTGGGAAATCCCGGATCCTTAATTGGAGATGATCAAATTTATAATGTTATTGTAACAGCCCATGCTTTCGTAATAATTTTTTTCATAGTTATACCTATTATAATTGGGGGATTTGGAAATTGATTAGTGCCACTAATATTAGGAGCACCAGATATAGCCTTCCCTCGAATAAATAACATGAGATTTTGACTTTTACCCCCTTCCTTGACCCTTCTCCTAATAAGTAGAATAGTTGAAAAAGGGGCAGGGACAGGTTGAACAGTTTACCCTCCGCTGTCATCAGGAATCGCTCACAGAGGGGCATCAGTGGACCTAGCTATTTTTAGACTTCATTTAGCAGGAATTTCATCAATTTTAGGAGCAGTAAATTTTATTACAACAATTATTAATATACGATCAGTAGGGATAACATTTGATCGAATACCCTTATTTGTATGATCAGTAGGAATTACAGCATTATTATTATTACTATCTTTACCAGTTTTAGCTGGAGCTATTACTATACTTCTAACAGATCGAAAATTTAAATACTTCTTTTTTTGATCCTGCCGGAGGGGGAGATCCTATTCTTTATCAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Carabus taedatus was found to be negative for Wolbachia.
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