Demaptera

November 21, 2022

Sample information
Picture
Location
Collection date 09/17/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Black with a light underbelly. wingless. 6 legs. Pincers on the abdomen. At time of collection: midday, 70-80 degrees F, sunny.
Putative identification Arthropoda Hexapoda Insecta Dermaptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Following the protocol provided by the Wolbachia project. We used a 10kb DNA ladder. We did not properly pipette the arthropod DNA into the well, which likely resulted in the 600 bp Arthropod DNA not showing up.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We did not properly pipette the arthropod DNA into the well, so we do not know if the DNA not showing up was caused by that or another error, as such, it is unclear if we found a lack of Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post