Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Formica aserva– Red Ant- Decaying log near trail in brush with colony
Description: dark red shiny Body length: 0.3cm Found by Danny Ahlstrom UTM Coordinates: 12T 0491458 4797119 Arthropod: + Wolbachia: + |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica aserva |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA Annotated protocol in Wolbachia project Lab2: DNA Extraction Edward’s Buffer |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | We tested and retested our sample and then re did the protocol and did the PCR again and sent it to the Wolbachia Project Lab. It was sequenced and then confirmed on Blast as a match. |
| Wolbachia 16S sequence |
>Wol19 GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
>Arthropod19_CO1F TCTATAAGTATAATTATTCGTTTAGAATTAGGCTCATCAAATTCATTAATCAATAATGATCAAATTTATAACTCTTTAGTAACTAATCATGCTTTTATCATAATTTTTTTTATAGTAATACCATTTATAATTGGAGGATTTGGAAATTTTCTAATTCCTTTAATATTAGGATCTCCAGATATAGCTTACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCCTCCATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGTACAGGTACAGGATGAACTATTTATCCTCCTTTAGCTTCAAATATTTTTCATAATGGCCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAGGTATATCATCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTTGTATGATCAATTTTAATCACAGCTATCTTACTTCTATTATCCTTACCTGTTTTAGCCGGAGCTATTACTATACTATTAACCGATCGAAATTTAAATACTTCA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Formica aserva was found to be postive for Wolbachia. |
Water Boatman