|Captive / Cultivated?||Wild-caught|
|Group||Essex North Shore Agricultural & Technical School|
Dead. Black, and similarly shaped to a carpenter ant.
|Putative identification||Hexapoda Insecta Hymenoptera Formicidae Crematogaster|
|Extraction kit||Monarch DNA extraction (NEB)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
Isolated DNA, made controls for both genomic DNA and positive/negative controls for Wolbachia, performed PCR, performed gel electrophoresis
|Explanation of confidence level||
There is a tiny band in where the negative control should be but it could be the fact that there was some runoff into the lane from when the gel was loaded.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Crematogaster was found to be postive for Wolbachia.|