Acrobat Ant

Sample information

Photos by: Jennifer Baker
Collection date
Captive / Cultivated? Wild-caught
Group Essex North Shore Agricultural & Technical School

Dead. Black, and similarly shaped to a carpenter ant.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Crematogaster


Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Isolated DNA, made controls for both genomic DNA and positive/negative controls for Wolbachia, performed PCR, performed gel electrophoresis


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

There is a tiny band in where the negative control should be but it could be the fact that there was some runoff into the lane from when the gel was loaded.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Crematogaster was found to be postive for Wolbachia.
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