Estigmene acrea caterpillar 1

September 7, 2023

Sample information
Picture
Photos by: Shreyan M., Max B., Charlie B.
Location
Collection date 10/30/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Multiple species feeding in the same location
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Erebidae Estigmene Estigmene acrea

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Caterpillar was cut into six slices. All six duplicates were tested for Wolbachia.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Estigmene acrea was found to be negative for Wolbachia.
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