Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Multiple species feeding in the same location |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera Erebidae Estigmene Estigmene acrea |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Caterpillar was cut into six slices. All six duplicates were tested for Wolbachia. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Estigmene acrea was found to be negative for Wolbachia. |
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