Pyrrharctia isabella caterpillar

September 7, 2023

Sample information
Picture
Photos by: Shreyan M., Max B., Charlie B.
Location
Collection date 10/09/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Multiple individuals inhabiting the same area
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Erebidae Pyrrharctia Pyrrharctia isabella

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Cut caterpillar into six sections from head to posterior, performed PCR and ran gel on each duplicate.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Pyrrharctia isabella was found to be postive for Wolbachia.
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