|Captive / Cultivated?||Wild-caught|
|Group||Georgia Southern University|
The insect was collected from underneath a log. On the day of collection it was cold, windy and overcast.
|Putative identification||Hexapoda Insecta Coleoptera|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
DNA extraction kit of in-house reagents was used
|Explanation of confidence level||
Upon doing research from the first couple of class periods I knew that my insect was not going to have Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Coleoptera was found to be negative for Wolbachia.|