NESACBSp23-8-MG Odonata

April 10, 2023

Sample information
Picture
Photos by: Makayla G
Location No map location data available.
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Aquatic environment

 
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls were correct and the Wolbachia gene was not found
Wolbachia 16S sequence
Arthropod COI sequence
AACAGCTCTTAGAGTTTTAATTCGAATCGAATTacggccAACCAGGATCT TTAATTGGAGATGATCAAATTTATAATGTTATTGTTACTGCTCACGCATT CGTTATAATTTTCTTCATAGTTATGCCTATTATAATTGGTGGCTTTGGAA ACTGGTTAGTTCCTTTAATATTAGGAGCCCCAGATATAGCCTTCCCCCGT CTTAATAATATAAGTTTTTGATTACTTCCTCCCTCCTTCACATTACTTTT ATCTAGAAGTATAGTAGAAAGAGGAGCAGGAACAGGATGAACTGTATATC CTCCATTGGCAGGAGCTATTGCACATGCAGGAGCATCTGTAGATTTAACT ATTTTCTCGTTACACTTAGCAGGAGTATCCTCCATTCTAGGAGCTATTAA TTTTATTACAACAGTAATCAATATAAAATCCCCTGGGATGAAGCTGGATC AAATACCTCTTTTTGTATGAGCAGTAGTAATTACTGCAGTATTATTACTT TTATCCCTTCCTGTATTGGCAGGAGCTATTACTATACTTTTAACAGATCG TAatATCAACACTTCATTCTTCGACCCAGCAGGGGGAGGAGATCCAATTT TATATCAGCACTTATTTTGATTTTTTGGTCACCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Odonata was found to be negative for Wolbachia.
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