DEMACASP23-12 Collembola

April 10, 2023

Sample information
Picture
Photos by: Nadia O.
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insect was collected in a grassy area in front of the Academic Success Building on the Armstrong Campus. Collection method was done with a sweep net. And the weather conditions were Sunny, Very Windy, and 59 degrees outside.
Putative identification Arthropoda Entognatha

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls were to be expected and there is no sign of contamination within the DNA samples.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Entognatha was found to be negative for Wolbachia.
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