DEMACASp23-11 Hymenoptera

April 10, 2023

Sample information
Picture
Photos by: Katie N.
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • 1:20 PM
  • found on the grass between the Student Success Complex and Student Recreational Center of the Armstrong campus
  • Sunny 57°F
  • 13 mph wind
  • Method: sweeping net
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident because when analyzing the results of the Gel Electrophoresis, there is no band shown for the Wolbachia gene in my specimen. This means that there was no Wolbachia detected and that my insect was not infected with Wolbachia. My controls worked properly, so I know that the results were not contaminated.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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