Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The collection method was a sweep net. The habitat contained tall grass and a damp ground. The weather was windy, sunny, and chilly outside. The insect was collected in the Science Center parking lot in the open field located in the Armstrong campus. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I correctly performed all of the steps in the Wolbachia project. There was not a reason for concern in any areas of the steps/process. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman