DEMACASp23-3; Diptera

April 10, 2023

Sample information
Picture
Photos by: Cailey E.
Location No map location data available.
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The collection method was a sweep net. The habitat contained tall grass and a damp ground. The weather was windy, sunny, and chilly outside. The insect was collected in the Science Center parking lot in the open field located in the Armstrong campus.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I correctly performed all of the steps in the Wolbachia project. There was not a reason for concern in any areas of the steps/process.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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