NESACBSp23-1 Diptera

April 10, 2023

Sample information
Picture
Photos by: Isabella S
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The Diptera was collected on 1/25/23 at around 22:30 to 23:00. The location was the Regis Way neighborhood in Richmond Hill GA. The environment/ habitat was a room temperature kitchen. The collection method was placing half of a tangerine on the counter and waiting for an insect to approach. When a fruit fly got on the tangerine, I quickly placed it into a bag.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The results for NESACBSp23-1 Diptera were inconclusive, therefore if the sample was positive it is still unknown. Also, there were mistakes in the experiment which reduces my confidence level further. Whether the sample was positive or negative, there is a lot of uncertainty. However, I am confident that the DNA was lost and that is why it is unknown. However, I have low confidence agreeing with it being positive for Wolbachia. Also, according to local collection the percentage of infected insects with the Order Diptera was 20%.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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