Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The Diptera was collected on 1/25/23 at around 22:30 to 23:00. The location was the Regis Way neighborhood in Richmond Hill GA. The environment/ habitat was a room temperature kitchen. The collection method was placing half of a tangerine on the counter and waiting for an insect to approach. When a fruit fly got on the tangerine, I quickly placed it into a bag. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
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| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The results for NESACBSp23-1 Diptera were inconclusive, therefore if the sample was positive it is still unknown. Also, there were mistakes in the experiment which reduces my confidence level further. Whether the sample was positive or negative, there is a lot of uncertainty. However, I am confident that the DNA was lost and that is why it is unknown. However, I have low confidence agreeing with it being positive for Wolbachia. Also, according to local collection the percentage of infected insects with the Order Diptera was 20%. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | |
Water Boatman