NESACKSp23-7ps

April 13, 2023

Sample information
Picture
Photos by: NESACKSp23-7ps Hemiptera
Location
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

26 Jan 23

10:00am

bush with pink flowers

Georgia Southern Campus

Stick and sheet
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction Kit of in house reagent was used

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I did not think my insect would be positive for Wolbachia. Because it had a low percentage for local data. Only 25 percent of Hemiptera are infected in Savannah,GA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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