Sample information |
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Picture |
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Location | |
Collection date | 09/05/2022 |
Captive / Cultivated? | Wild-caught |
Group | College of Eastern Idaho |
Observations | Woodland Butterfly- Long blades of green and yellow grass in open area Description: Tan with orange markings Body length: 1.78 cm. Found by Charyn Brooks |
Putative identification | Arthropoda Hexapoda Insecta Lepidoptera Hesperiidae Ochlodes Ochlodes sylvanoides |
Methods |
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Extraction kit | Edwards Buffer |
DNA extraction location | Abdomen |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
ATTTGAGCAGGAATATTAGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCAGGATCTTTAATTGGCGATGACCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACTGGTTGAACAGTATATCCTCCTTTATCTTCTAATATTGCTCACCAAGGATCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCTGGTATTTCATCTATTCTAGGAGCTATTAATTTTATTACAACAATTATCAATATACGAATTAAAAACTTATCATTTGATCAAATACCCTTATTTGTATGATCAGTAGGTATTACAGCATTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACAATATTACTTACTGATCGAAATTTAAATACTTCTTTTTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTT
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Ochlodes sylvanoides was found to be negative for Wolbachia. |