Ochlodes sylvanoides- Woodland Skipper Butterfly

April 27, 2023

Sample information
Picture
Photos by: Chase Hartzell and Heather Ahlstrom
Location
Collection date 09/05/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Woodland Butterfly- Long blades of green and yellow grass in open area

Description: Tan with orange markings

Body length: 1.78 cm. Found by Charyn Brooks
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Hesperiidae Ochlodes Ochlodes sylvanoides

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
ATTTGAGCAGGAATATTAGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCAGGATCTTTAATTGGCGATGACCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACTGGTTGAACAGTATATCCTCCTTTATCTTCTAATATTGCTCACCAAGGATCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCTGGTATTTCATCTATTCTAGGAGCTATTAATTTTATTACAACAATTATCAATATACGAATTAAAAACTTATCATTTGATCAAATACCCTTATTTGTATGATCAGTAGGTATTACAGCATTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACAATATTACTTACTGATCGAAATTTAAATACTTCTTTTTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Ochlodes sylvanoides was found to be negative for Wolbachia.
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