Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Woodland Butterfly- Long blades of green and yellow grass in open area Description: Tan with orange markings Body length: 1.78 cm. Found by Charyn Brooks |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera Hesperiidae Ochlodes Ochlodes sylvanoides |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATTTGAGCAGGAATATTAGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCAGGATCTTTAATTGGCGATGACCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACTGGTTGAACAGTATATCCTCCTTTATCTTCTAATATTGCTCACCAAGGATCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCTGGTATTTCATCTATTCTAGGAGCTATTAATTTTATTACAACAATTATCAATATACGAATTAAAAACTTATCATTTGATCAAATACCCTTATTTGTATGATCAGTAGGTATTACAGCATTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACAATATTACTTACTGATCGAAATTTAAATACTTCTTTTTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Ochlodes sylvanoides was found to be negative for Wolbachia. |
Water Boatman