Prosternon bombycinum- Beetle Grub

April 27, 2023

Sample information
Picture
Photos by: Chase Hartzell and Heather Ahlstrom
Location
Collection date 09/05/2022
Captive / Cultivated?
Group College of Eastern Idaho
Observations

Beetle Grub- Under a log in brush along dirt path

Description: tan and yellow color

Body length: 2.03 cm. Found by Charyn Brooks
Putative identification Arthropoda Hexapoda Insecta Coleoptera Elateridae Prosternon Prosternon bombycinum

Methods
Extraction kit Edwards Buffer
DNA extraction location Other
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain
Gel images
Protocol notes

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
GATATTAGGAACTTCATTAAGATTATTAATTCGGGCAGAACTAGGAAATCCAGGATCATTAATCGGAAATGACCAAATTTACAACGTTATTGTCACAGCACATGCTTTCATTATAATTTTCTTCATAGTTATACCAATCATAATTGGGGGATTCGGAAATTGATTAGTACCCCTAATACTAGGAGCACCTGATATGGCATTCCCACGAATAAATAACATAAGATTCTGATTTCTACCCCCATCACTATCTCTTCTATTAATAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGCTGAACAGTTTATCCCCCCCTCTCAGCAAACATTGCCCATAGTGGATCTTCAGTTGATTTAGCCATTTTCAGTCTACATTTAGCAGGAATCTCATCAATCTTAGGAGCAGTAAATTTCATTTCAACTGTTATTAATATACGAACAACAGGAATCACATTCGACCGAATACCTTTATTTGTATGAGCCGTAGCAATTACAGCTCTTCTTCTATTATTATCTTTACCTGTATTAGCAGGAGCCATCACAATATTACTTACTGACCGAAATCTAAATACATCATTTTTTGATCCTGCAGGAGGAGGAGATCCAATCCTATACCAACACCTATTCT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Prosternon bombycinum was found to be negative for Wolbachia.
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