Drunella coloradensis- Stonefly Nymph

April 27, 2023

Sample information
Picture
Photos by: Chase Hartzell and Heather Ahlstrom
Location
Collection date 09/05/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Stonefly Nymph- Under rock in the river

Description: Brown with a red hue

Body length: 1.27 cm. Found by Danny Ahlstrom
Putative identification Arthropoda Hexapoda Insecta Ephemeroptera Ephemerellidae Drunella Drunella coloradensis

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
GGACTTCGTTGAGGTTACGCATCCAGGCAGAACTAGGGCAACCTGGGTCAGTNNTGGGANATGACCGNNCNANAATGGCATTGTAACGNCCATGCCTTCATTATAATTTTCTTTATGGTAATACCTATTATAATTGGGGGATTCGGGAATTGATTAGTTCCGTTAATGCTTGGTGCCCCCGATATAGCATTTCCCCGAATGAACAATATAAGCTTTTGGTTGTTACCTCCTGCCCTAACGTTATTATTGGCTAGAAGTATAGTGGAAAGAGGGGCAGGAACCGGTTGGACAGTTTACCCCCCCTTGGCTTCCGGCATTGCCCATGCTGGGGGATCTGTAGATCTTGCTATCTTTTCGCTTCACTTAGCGGGAGTCTCCTCTATTCTCGGAGCAGTAAATTTTATTACAACCACCATTAATATACGAGCCAGAGGAATAACTATGGACCGAATTCCCCTATTTGTATGGTCCGTTTTAATTACCGCAATCCTCCTTCTTCTCTCCTTACCGGTCCTTGCTGGGGCTATTACTATGCTTCTTACAGACCGTAACTTAAATACCTCGTTCTTTGACCCGGCGGGTGGGGGAGACCCTATTTTATACCAACATCT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Drunella coloradensis was found to be negative for Wolbachia.
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