Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Stonefly Nymph- Under rock in the river Description: Brown with a red hue Body length: 1.27 cm. Found by Danny Ahlstrom |
| Putative identification | Arthropoda Hexapoda Insecta Ephemeroptera Ephemerellidae Drunella Drunella coloradensis |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GGACTTCGTTGAGGTTACGCATCCAGGCAGAACTAGGGCAACCTGGGTCAGTNNTGGGANATGACCGNNCNANAATGGCATTGTAACGNCCATGCCTTCATTATAATTTTCTTTATGGTAATACCTATTATAATTGGGGGATTCGGGAATTGATTAGTTCCGTTAATGCTTGGTGCCCCCGATATAGCATTTCCCCGAATGAACAATATAAGCTTTTGGTTGTTACCTCCTGCCCTAACGTTATTATTGGCTAGAAGTATAGTGGAAAGAGGGGCAGGAACCGGTTGGACAGTTTACCCCCCCTTGGCTTCCGGCATTGCCCATGCTGGGGGATCTGTAGATCTTGCTATCTTTTCGCTTCACTTAGCGGGAGTCTCCTCTATTCTCGGAGCAGTAAATTTTATTACAACCACCATTAATATACGAGCCAGAGGAATAACTATGGACCGAATTCCCCTATTTGTATGGTCCGTTTTAATTACCGCAATCCTCCTTCTTCTCTCCTTACCGGTCCTTGCTGGGGCTATTACTATGCTTCTTACAGACCGTAACTTAAATACCTCGTTCTTTGACCCGGCGGGTGGGGGAGACCCTATTTTATACCAACATCT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Drunella coloradensis was found to be negative for Wolbachia. |
Water Boatman