Steatoda Grossa Wolbachia Test

May 11, 2023

Sample information
Picture
Photos by: Valentina V., Kevin L., Marianna McD., Jaylen S.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

Small in size (1in. tall), abdomen and limbs hairy, spinneret not visible, abdomen black and cephalothorax is caramel brown. Produced a visibly “tangled” cobweb at the end where the spinneret would be (a clump of cobweb we assumed was just dust.)
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We were not confident at all because when we checked our gel, the well with the specimen DNA was empty with no bands, faint or not.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Steatoda was found to be negative for Wolbachia.
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