Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/06/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | Small in size (1in. tall), abdomen and limbs hairy, spinneret not visible, abdomen black and cephalothorax is caramel brown. Produced a visibly “tangled” cobweb at the end where the spinneret would be (a clump of cobweb we assumed was just dust.) |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We were not confident at all because when we checked our gel, the well with the specimen DNA was empty with no bands, faint or not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Steatoda was found to be negative for Wolbachia. |
Water Boatman