Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/26/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collected with bug net and beat and stick method in the morning when it was cold. Insect appears green with two pairs of wings. |
| Putative identification | Arthropoda Hexapoda Insecta Neuroptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | This was my first time doing a lab, throughout the exercises my confidence grew until the negative control was loaded wrongly. However I am quite confident in my results because of the research I’ve done in the past labs. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Neuroptera was found to be negative for Wolbachia. |
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus