Thrive Scholars – Summer 2023 – Pillbug – The Blue Gloves

August 9, 2023

Sample information
Picture
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Followed the protocol. The top lanes are arthropod primers and the bottom lanes are Wolbachia primers. The sample is in lane 2.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The confidence level is medium because the bars were streaked in the sample results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be postive for Wolbachia.
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