Thrive Scholars – Pill bug – LA Team

August 9, 2023

Sample information
Picture
Photos by: Kelly N., Kimberly N., Natalee R., Desiree R.
Location
Collection date 08/07/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations

Regular Pillbug
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website.

Top gel: arthropod DNA primer

Bottom gel: wolbachia DNA primer

sample in the first lane for both gels

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was clearly no wolbachia DNA fragments present, but the top half of the gel with the arthropod primer makes us doubt the validity of the entire experiment since it was also unclear if there was arthropod DNA in the sample.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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