|Captive / Cultivated?
|Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
|Standard electrophoresis system
We followed the protocol on the website. The top lanes use the arthropod primer and the bottom lanes use the wolbachia primer.
|Explanation of confidence level
The pill bug is Wolbachia negative because there is no DNA band on Lane 3 of the Wolbachia gel. However, the confidence level is low because there is no DNA on lane 3 on arthropod gel even though the specimen is an arthropod. This suggests that there may be cross-contamination during the gel electrophoresis. There is a low confidence because the gel has a streaky band.
|Wolbachia 16S sequence
|Arthropod COI sequence
|The Armadillidium was found to be negative for Wolbachia.