Thrive Scholars- Pillbug- DATeam

August 9, 2023

Sample information
Picture
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website. The top lanes use the arthropod primer and the bottom lanes use the wolbachia primer.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The pill bug is Wolbachia negative because there is no DNA band on Lane 3 of the Wolbachia gel. However, the confidence level is low because there is no DNA on lane 3 on arthropod gel even though the specimen is an arthropod. This suggests that there may be cross-contamination during the gel electrophoresis. There is a low confidence because the gel has a streaky band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium was found to be negative for Wolbachia.
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