Crane fly

Sample information

Picture
Photos by: Damian H
Location
Collection date 11/02/2020
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Collected on misty morning from car door frame

Putative identification Hexapoda Insecta Diptera Tipulidae Tipula

Methods

Extraction kit Instagene Matrix
DNA extraction location Abdomen
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
We first ran duplex PCR reaction (with both COI and Wolbachia primers) and then ran separate PCR reactions with just COI and just Wolbachia primers

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

High confidence level, the gel showed now Wolbachia band at the corresponding marker on the control lane

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary The Tipula was found to be negative for Wolbachia.
Report Inappropriate Post