Aedes aegypti

November 26, 2023

Sample information
Picture
Location
Collection date 09/18/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

The Mosquito wa very aggressive, coming back to bite me, despite my almost capturing it several times
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction

The liquid was not viscous at all because the mosquito was extremely small. The size also caused difficulty in grinding it up.

We did not make any noticeable mistakes

 

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AGAATTTTAATTCGTGCTGAACTTAGCCACCCTGGTATATTTATTGGGAATGACNNNATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hexapoda was found to be negative for Wolbachia.
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