Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/18/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | The Mosquito wa very aggressive, coming back to bite me, despite my almost capturing it several times |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA Extraction The liquid was not viscous at all because the mosquito was extremely small. The size also caused difficulty in grinding it up. We did not make any noticeable mistakes
|
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
AGAATTTTAATTCGTGCTGAACTTAGCCACCCTGGTATATTTATTGGGAATGACNNNATT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hexapoda was found to be negative for Wolbachia. |
Water Boatman