Cicada (Tibicen tibicen)

November 26, 2023

Sample information
Picture
Photos by: Lucinda M
Location
Collection date 09/09/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Medium-size, black exoskeleton, medium mandibles on head
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: The arthropod was pretty easy to grind up, though removing reproductive organs was a challenge as the arthropod was hollow. No errors were made

Gel Electrophoresis Arthropod CO1: slight error with pipetting, but no harm was made to the experiment. Arthropod line showed up

Gel Electrophoresis Wolbachia : Lane 1 (DNA ladder), lane 2 (B1), lane 3 (B2), lane 4 (B3), lane five (B4) lane 6 (WOL+), lane 7 (WOL -), lane 8 (water)

The controls were successful

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The procedure was followed very closely and there was not even a faint line present, the gel was completely Wolbachia-negative.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Hemiptera was found to be negative for Wolbachia.
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