Katydid

November 26, 2023

Sample information
Picture
Photos by: Wren G.
Location
Collection date 09/13/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

4 wings, 6 legs, large mandibles, long antenna, hooks on back-legs, bright green and brown, cryptic coloration (resembles leaf), approximately 4-5 cm, moved by jumping, attracted to light, lacked long ovipositor (reason to believe it is a male specimen)
Putative identification Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The katydid did not test positive for arthropod DNA. This could be for several reasons, and I will redo part of the lab to see if I can extract DNA.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Likely, the primers did not stick to the katydid’s DNA leading to no data during the Arthropod gel electrophoresis; however, it did not show positive for Wolbachia, which it would have if it was positive regardless of the primers sticking or not. to be safe, I put my confidence level as low because of the inconclusively.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tettigoniidae was found to be negative for Wolbachia.
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