Katydid

Sample information
Picture	Photos by: Wren G.
Location
Collection date	09/13/2023
Captive / Cultivated?	Wild-caught
Group	Edmund Burke School
Observations	4 wings, 6 legs, large mandibles, long antenna, hooks on back-legs, bright green and brown, cryptic coloration (resembles leaf), approximately 4-5 cm, moved by jumping, attracted to light, lacked long ovipositor (reason to believe it is a male specimen)
Putative identification	Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	The katydid did not test positive for arthropod DNA. This could be for several reasons, and I will redo part of the lab to see if I can extract DNA.
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	Likely, the primers did not stick to the katydid’s DNA leading to no data during the Arthropod gel electrophoresis; however, it did not show positive for Wolbachia, which it would have if it was positive regardless of the primers sticking or not. to be safe, I put my confidence level as low because of the inconclusively.
Wolbachia 16S sequence	N/A BLAST at The Wolbachia Project BLAST at NCBI
Arthropod COI sequence	N/A BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Tettigoniidae was found to be negative for Wolbachia.

The Wolbachia Project Database