Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Scarborough High School |
| Observations | Found the specimen in a parking lot behind the El Rayo restaurant, near the high school. There were large bushy trees and weeds that had blossoms that were white. I captured it while it was resting on one of the blossoms. There was lots of trash around. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Syrphidae Toxomerus Toxomerus occidentalis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | H20 got contaminated probably by a pipette tip |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Our H20 had a slight line which could show contamination within the agarose. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Toxomerus occidentalis was found to be negative for Wolbachia. |
Water Boatman