Sample information |
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Picture |
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Location | |
Collection date | 09/20/2023 |
Captive / Cultivated? | Wild-caught |
Group | Scarborough High School |
Observations | The moth was found on a blade of grass in the courtyard of a school, in a suburban area. It was approximately 70 degrees and sunny. The insect is tan colored, with a long snout and black eyes. |
Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
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Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniPCR |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | All controls showed expected results, no known errors. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Lepidoptera was found to be negative for Wolbachia. |