Snout moth

December 15, 2023

Sample information
Picture
Photos by: Fiona M
Location
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

The moth was found on a blade of grass in the courtyard of a school, in a suburban area. It was approximately 70 degrees and sunny. The insect is tan colored, with a long snout and black eyes.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls showed expected results, no known errors.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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