Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Scarborough High School |
| Observations | The moth was found on a blade of grass in the courtyard of a school, in a suburban area. It was approximately 70 degrees and sunny. The insect is tan colored, with a long snout and black eyes. |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls showed expected results, no known errors. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman