Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/26/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Scarborough High School |
| Observations | Collected at the back of Scarborough High School in area with greenery, but found crawling on the cement around the greenery. It was approx. 70 degrees fahrenheit and partly cloudy.
|
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | We had some debris fall into the top of the agorose gel when it was closed. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | We are medium confiend because of the faintness of the lines, but our controls reflected the right results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be negative for Wolbachia. |
Thrip – Phlaeothripidae Leptothrips sp. 21
MACALF24-SB Dermaptera
Thrip – Phlaeothripidae Leptothrips sp. 19