Spiny Orb Weaver

October 1, 2023

Sample information
Picture
Photos by: Ryan S
Location
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

I found the Spiny Orb Weaver while it was on its web. The time of the catch was about 2pm, it was sunny with a temperature of around 83°F. The web was connected to flora on top of a wooden fence. An image of the area that it was collected is attached.

Putative identification Arthropoda Chelicerata Arachnida Araneae Araneidae Gasteracantha Gasteracantha cancriformis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Used 6X loading Dye

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected. The only problem that occurred was that there was no amplification of the arthropod DNA. Only Wolbachia PCR amplification showed up on the gel.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Gasteracantha cancriformis was found to be negative for Wolbachia.
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