Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/12/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Palm Beach Atlantic University |
| Observations | I found the Spiny Orb Weaver while it was on its web. The time of the catch was about 2pm, it was sunny with a temperature of around 83°F. The web was connected to flora on top of a wooden fence. An image of the area that it was collected is attached. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Araneidae Gasteracantha Gasteracantha cancriformis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | Used 6X loading Dye |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of my controls worked as expected. The only problem that occurred was that there was no amplification of the arthropod DNA. Only Wolbachia PCR amplification showed up on the gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Gasteracantha cancriformis was found to be negative for Wolbachia. |
Water Boatman