Sample information
Picture	Photos by: Sonia K.
Location
Collection date	08/29/2023
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	Found in a big clump, black, small
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tetramorium
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Tetramorium was found to be postive for Wolbachia.