Brickwork Woodlouse

March 15, 2024

Sample information
Picture
Photos by: Carys H
Location
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

There were many brickwork woodlouse in the front lawn near a pothole. They had long antennae and a snakeskin-like pattern but with horizontal lines. The weather was about 80 degrees F at the time.
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Not sure if I dissected the woodlouse correctly, it was small but large enough to still dissect it so I couldn’t properly see the reproductive organs.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

A very faint line showed up on the Arthropod PCR after completing gel electrophoresis, but I still think I didn’t get enough DNA from the extraction process, so the arthropod could’ve had Wolbachia, but I didn’t have enough DNA to prove it.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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