Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/29/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | This ant was found on the pavement next to a big ant colony near the lawn. It is black and small with an antennae. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tetramorium |
Methods |
|
| Extraction kit | |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | My data doesn’t seem completely wrong because my arthropod DNA showed up, so I’m kind of confident that the procedure went right and my arthopod is wolbachia negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Tetramorium was found to be negative for Wolbachia. |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus