Cricket

October 17, 2023

Sample information
Picture
Photos by: Katie M
Location
Collection date 09/07/2023
Captive / Cultivated? Captive / Cultivated
Group Palm Beach Atlantic University
Observations

I collected only one cricket from a container containing many other crickets. The crickets were living in an environment with mulch/straw. I collected this cricket from PetSmart in West Palm Beach, FL 33401 on September 7, 2023 at 3:00pm.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Gryllidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: I removed the exoskeleton and only dissected a piece of the lower abdomen out. I made sure to crush the cricket abdomen really well.
Gel Electrophoresis:
– used a 2% agarose gel
– used 4 ul of 6X loading dye
– used 10 ul of 1 Kb plus DNA ladder
– used 20 ul samples to load the DNA samples and the water
– 100 V used to run gel
Analysis: My controls worked properly. My sample had one band displaying the CO1 gene. DNA extraction was done properly.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected. I did not have any problems with the protocol. The bands appeared as they should have; therefore, I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Gryllidae was found to be negative for Wolbachia.
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